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By Richard J. Geider, Bruce A. Osborne (auth.)

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The stable carbon isotope 13C can substitute for 14C under some conditions and measurements of natural abundance of 13C relative to 12C (the carbon-isotope ratio a13C) have provided considerable insight into photosynthetic mechanisms and form(s) of inorganic carbon assimilated by plants. Measurements of a13c have also been used to elucidate the physical and biochemical rate-limiting steps in photosynthetic carbon fixation. The stable oxygen isotope 180 has been useful in evaluating the roles of respiration, photorespiration, and the Mehler reaction in illuminated plants.

3 atm (330 ILmol 02·dm-3). Kaplan and Berry (1981) found that decreases in the PQ of Chlamydomonas reinhardtii were correlated with increases in the excretion of glycolate, which is one of the products of ribulose bisphosphate carboxylase-oxygenase (RUBISCO) oxygenase activity. In contrast, Bidwell and McLachlan (1985) found no evidence for photorespiration except in an atmosphere with simultaneously elevated O 2and very low CO2(50 X 10-4 cm3·dm -3), although an 02-sensitive wound respiration that could potentially be confused with photorespiration was observed.

Polycarbonate* bottles should be used for incubating samples. 25N nitric acid for 3 days, and rinsed with Milli-Q water. 6. 3 g·dm- 3 sodium carbonate solutions. Working solutions should be stored in acid-cleaned Teflon bottles. 7. All pipette tips used to dispense 14C solutions should be rinsed three times in 2molm- 3 redistilled nitric acid, and six times with Milli-Q water prior to use. 25 cm- 3 ) of 14C stock solutions are used to further reduce potential contamination. 8. Plastic gloves and lab coats should be worn at all times when injecting samples.

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